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Collagen alignment along the long axis of physical boundary is maintained despite YAP and ROCK inhibition. A) PLM imaging and color map analysis of collagen. B) Collagen intensity and coherency graphs. C) Immunohistochemistry for collagens I and II. Nuclei stained with hematoxylin. D) Heat map of <t>Brillouin</t> frequency shift. E) Brillouin frequency shift measurement. An ordinary one-way analysis of variance (ANOVA) with Tukey's multiple comparisons was used for statistical analysis (n = 3). Significance levels: ns = not significant, ∗∗∗∗p < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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Collagen alignment along the long axis of physical boundary is maintained despite YAP and ROCK inhibition. A) PLM imaging and color map analysis of collagen. B) Collagen intensity and coherency graphs. C) Immunohistochemistry for collagens I and II. Nuclei stained with hematoxylin. D) Heat map of <t>Brillouin</t> frequency shift. E) Brillouin frequency shift measurement. An ordinary one-way analysis of variance (ANOVA) with Tukey's multiple comparisons was used for statistical analysis (n = 3). Significance levels: ns = not significant, ∗∗∗∗p < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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Collagen alignment along the long axis of physical boundary is maintained despite YAP and ROCK inhibition. A) PLM imaging and color map analysis of collagen. B) Collagen intensity and coherency graphs. C) Immunohistochemistry for collagens I and II. Nuclei stained with hematoxylin. D) Heat map of <t>Brillouin</t> frequency shift. E) Brillouin frequency shift measurement. An ordinary one-way analysis of variance (ANOVA) with Tukey's multiple comparisons was used for statistical analysis (n = 3). Significance levels: ns = not significant, ∗∗∗∗p < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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Collagen alignment along the long axis of physical boundary is maintained despite YAP and ROCK inhibition. A) PLM imaging and color map analysis of collagen. B) Collagen intensity and coherency graphs. C) Immunohistochemistry for collagens I and II. Nuclei stained with hematoxylin. D) Heat map of <t>Brillouin</t> frequency shift. E) Brillouin frequency shift measurement. An ordinary one-way analysis of variance (ANOVA) with Tukey's multiple comparisons was used for statistical analysis (n = 3). Significance levels: ns = not significant, ∗∗∗∗p < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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Collagen alignment along the long axis of physical boundary is maintained despite YAP and ROCK inhibition. A) PLM imaging and color map analysis of collagen. B) Collagen intensity and coherency graphs. C) Immunohistochemistry for collagens I and II. Nuclei stained with hematoxylin. D) Heat map of <t>Brillouin</t> frequency shift. E) Brillouin frequency shift measurement. An ordinary one-way analysis of variance (ANOVA) with Tukey's multiple comparisons was used for statistical analysis (n = 3). Significance levels: ns = not significant, ∗∗∗∗p < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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Collagen alignment along the long axis of physical boundary is maintained despite YAP and ROCK inhibition. A) PLM imaging and color map analysis of collagen. B) Collagen intensity and coherency graphs. C) Immunohistochemistry for collagens I and II. Nuclei stained with hematoxylin. D) Heat map of <t>Brillouin</t> frequency shift. E) Brillouin frequency shift measurement. An ordinary one-way analysis of variance (ANOVA) with Tukey's multiple comparisons was used for statistical analysis (n = 3). Significance levels: ns = not significant, ∗∗∗∗p < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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Collagen alignment along the long axis of physical boundary is maintained despite YAP and ROCK inhibition. A) PLM imaging and color map analysis of collagen. B) Collagen intensity and coherency graphs. C) Immunohistochemistry for collagens I and II. Nuclei stained with hematoxylin. D) Heat map of <t>Brillouin</t> frequency shift. E) Brillouin frequency shift measurement. An ordinary one-way analysis of variance (ANOVA) with Tukey's multiple comparisons was used for statistical analysis (n = 3). Significance levels: ns = not significant, ∗∗∗∗p < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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Collagen alignment along the long axis of physical boundary is maintained despite YAP and ROCK inhibition. A) PLM imaging and color map analysis of collagen. B) Collagen intensity and coherency graphs. C) Immunohistochemistry for collagens I and II. Nuclei stained with hematoxylin. D) Heat map of <t>Brillouin</t> frequency shift. E) Brillouin frequency shift measurement. An ordinary one-way analysis of variance (ANOVA) with Tukey's multiple comparisons was used for statistical analysis (n = 3). Significance levels: ns = not significant, ∗∗∗∗p < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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Collagen alignment along the long axis of physical boundary is maintained despite YAP and ROCK inhibition. A) PLM imaging and color map analysis of collagen. B) Collagen intensity and coherency graphs. C) Immunohistochemistry for collagens I and II. Nuclei stained with hematoxylin. D) Heat map of <t>Brillouin</t> frequency shift. E) Brillouin frequency shift measurement. An ordinary one-way analysis of variance (ANOVA) with Tukey's multiple comparisons was used for statistical analysis (n = 3). Significance levels: ns = not significant, ∗∗∗∗p < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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Image Search Results


Collagen alignment along the long axis of physical boundary is maintained despite YAP and ROCK inhibition. A) PLM imaging and color map analysis of collagen. B) Collagen intensity and coherency graphs. C) Immunohistochemistry for collagens I and II. Nuclei stained with hematoxylin. D) Heat map of Brillouin frequency shift. E) Brillouin frequency shift measurement. An ordinary one-way analysis of variance (ANOVA) with Tukey's multiple comparisons was used for statistical analysis (n = 3). Significance levels: ns = not significant, ∗∗∗∗p < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Journal: Materials Today Bio

Article Title: Embedded cell-only bioprinting to engineer structurally aligned meniscal fibrocartilage

doi: 10.1016/j.mtbio.2026.103308

Figure Lengend Snippet: Collagen alignment along the long axis of physical boundary is maintained despite YAP and ROCK inhibition. A) PLM imaging and color map analysis of collagen. B) Collagen intensity and coherency graphs. C) Immunohistochemistry for collagens I and II. Nuclei stained with hematoxylin. D) Heat map of Brillouin frequency shift. E) Brillouin frequency shift measurement. An ordinary one-way analysis of variance (ANOVA) with Tukey's multiple comparisons was used for statistical analysis (n = 3). Significance levels: ns = not significant, ∗∗∗∗p < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Measurements were acquired using an inverted Brillouin microscope (Discoverer, CellSense Technologies GmbH, Berlin, Germany) equipped with a 780 nm laser and a 20X air objective (Olympus, NA 0.50, UPlanFI).

Techniques: Inhibition, Imaging, Immunohistochemistry, Staining